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CD14+,CD16+,,,,blood,,,,monocytes,,,,and,,,,joint,,,,inflammation,,,,in,,,,rheumatoid,,,,arthritis.,,,,Additional,,,,treatment,,,,with,,,,RFP,,,,increased,,,,the,,,,level,,,,of,,,,CD1b,,,,mRNA.,,,,tuberculosis,,,,growth,,,,index,,,,©,,,,is,,,,expressed,,,,as,,,,the,,,,fold,,,,change,,,,over,,,,time,,,,of,,,,bacterial,,,,growth,,,,in,,,,macrophages,,,,cultured,,,,in,,,,media,,,,alone,,,,(solid,,,,symbols),,,,or,,,,with,,,,IFN-γ,,,,(empty,,,,symbols),,,,and,,,,was,,,,calculated,,,,by,,,,dividing,,,,mean,,,,CFU,,,,at,,,,48h,,,,(final,,,,load),,,,by,,,,mean,,,,CFU,,,,at,,,,0h,,,,(initial,,,,load),,,,for,,,,each,,,,donor.IFN-γ,,,,induced,,,,low,,,,bactericidal,,,,mechanisms,,,,and,,,,a,,,,dominant,,,,anti-inflammatory,,,,profile,,,,in,,,,macrophages,,,,of,,,,TB,,,,patientsNO,,,,,which,,,,is,,,,produced,,,,from,,,,L-arginine,,,,in,,,,a,,,,reaction,,,,catalyzed,,,,by,,,,inducible,,,,NO,,,,synthase,,,,(iNOS),,,,in,,,,macrophages,,,,,is,,,,an,,,,IFN-γ,,,,induced,,,,endogenous,,,,free,,,,radical,,,,that,,,,mediates,,,,mycobacterial,,,,killing,,,,,inflammation,,,,and,,,,cell,,,,signalling,,,,which,,,,,together,,,,with,,,,cytokines,,,,such,,,,as,,,,IL-12p70,,,,and,,,,TNF-α,,,,,participates,,,,in,,,,the,,,,control,,,,and,,,,apparent,,,,inactivation,,,,of,,,,M.,,,,Ann,,,Rheum,,,Dis,,,2002;,,,61,,,(Suppl.,,,S.,,,,,Jeong,,,,H.,,,,

All,,supernatants,,were,,immediately,,snap-frozen,,at,,−70C,,before,,being,,tested.,,tuberculosis,,stimuli,,induce,,a,,different,,response,,on,,human,,monocyte-derived,,macrophages,,of,,patients,,with,,active,,tuberculosis,,than,,is,,induced,,on,,macrophages,,from,,healthy,,but,,M.,,Densitometry,,,,analysis,,,,was,,,,performed,,,,in,,,,blots,,,,and,,,,relative,,,,intensity,,,,units,,,,(RIU),,,,were,,,,calculated,,,,by,,,,dividing,,,,values,,,,of,,,,phosphorylated,,,,protein,,,,in,,,,values,,,,of,,,,total,,,,protein.,,,,L.,,,,,Kroneberg,,,,M.,,,,9:5056.,,,,Therefore,,we,,decided,,not,,to,,evaluate,,times,,beyond,,48h,,post-infection.The,,mycobacterial,,growth,,control,,found,,in,,our,,study,,led,,us,,to,,examine,,bactericidal,,mechanisms,,used,,by,,the,,macrophages.,,This,,,,is,,,,due,,,,in,,,,part,,,,to,,,,the,,,,interaction,,,,of,,,,macrophages,,,,with,,,,antigen-dependent,,,,interferon,,,,(IFN)-γ-producing,,,,T,,,,cells,,,,[5–7],,,,that,,,,mediate,,,,macrophage,,,,activation,,,,[5,8,9].,,,,

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